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Journal of Traditional Chinese Medicine ›› 2021, Vol. 41 ›› Issue (3): 424-431.DOI: 10.19852/j.cnki.jtcm.2021.03.010

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Full composition granules of Huanglian (Rhizoma Coptidis) decrease the serum monocyte chemotactic protein-1 and connective tissue growth factor levels and inhibit kidney nuclear factor-κB expression in rats with high-fat diet-induced diabetes

GUO Yangzhi, DU Juan, JIANG Min, GUO Wei   

  1. Department of Traditional Chinese Medicine, Capital Medical University Affiliated Beijing Shijitan Hospital;Department of Obstetrics and Gynecology, Beijing Changping Hospital of Integrated Chinese and Western Medicine;Department of Traditional Chinese Medicine, the Mongolian Medical and Traditional Chinese Medicine Hospital of Wuhai;
  • Online:2021-06-03 Published:2021-06-03
  • Supported by:
    Supported by Beijing Traditional Chinese Medicine Science and Technology Project:Study on the Mechanism of High Dose Huanglian (Rhizoma Coptidis) in Treating Diabetic Nephropathy (No.QN2014-08)

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Abstract: OBJECTIVE: To investigate the efficacy of the full composition granules of Huanglian(Rhizoma Coptidis)(FGC) on the serum monocyte chemotactic protein-1(MCP-1) and connective tissue growth factor(CTGF) levels and kidney nuclear factor-κB(NF-κB) expression in rats with high-fat diet-induced diabetes.METHODS: Diabetes was induced in rats by feeding a high-fat chow combined with intravenous streptozotocin injection. Forty diabetic SpragueDawley rats were randomly assigned to a normal group(NG), model group(MG), irbesartan group(IG), and low-, middle-, and high-dosage FGC groups(LFGC, MFGC, HFGC), with eight rats per group. The IG rats received 31.25 mg·kg~(-1)·d~(-1) irbesartan tablets, whereas those in the LFGC, MFGC,and HFGC were administered 52, 312.5, and 625 mg·kg~(-1)·d~(-1) FGC, respectively. After 12 weeks,bodyweight(BW), left kidney weight(KW), hemoglobin A1c(HbA1c), serum creatinine(Scre), blood urea nitrogen(BUN), and serum MCP-1 and CTGF levels were determined, pathological changes of the kidney were recorded, and kidney NF-κB p65(A) expression was measured.RESULTS: The 24-h urine albumin and levels of HbA1c, Scre, BUN, and serum MCP-1 and CTGF were significantly increased in in the MG compared with the NG, as was the kidney NF-κB(p65) expression(P < 0.05). Furthermore, clear pathological changes in kidney fibrosis were observed in the MG rats. Following irbesartan and FGC administration,the 24-h urine albumin and the levels of HbA1c,Scre, and serum MCP-1 and CTGF were significantly decreased in FCG groups compared with those in the MG, which is in agreement with the change in the kidney NF-κB(p65) expression, whereas the similarly significant decrease only exist in 24-h urine albumin and the levels of serum CTGF after irbesartan administration. Hematoxylin-eosin(HE)staining results indicated that the fibrosis observed in the MG samples was alleviated through FGC treatment.CONCLUSION: FGC may alleviate potential kidney injury by decreasing the serum MCP-1 and CTGF levels and inhibiting NF-k B expression in diabetic nephropathy in rats with high-fat diet-induced diabetes.

Key words: Huanglian(Rhizoma Coptidis), diabetic nephropathies, chemokine CCL2, connective tissue growth factor, NF-kappa B

Cite this article

GUO Yangzhi, DU Juan, JIANG Min, GUO Wei. Full composition granules of Huanglian (Rhizoma Coptidis) decrease the serum monocyte chemotactic protein-1 and connective tissue growth factor levels and inhibit kidney nuclear factor-κB expression in rats with high-fat diet-induced diabetes[J]. Journal of Traditional Chinese Medicine, 2021, 41(3): 424-431.