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Journal of Traditional Chinese Medicine ›› 2021, Vol. 41 ›› Issue (3): 360-366.DOI: 10.19852/j.cnki.jtcm.2021.03.003

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Tiaobu Feishen therapy inhibits inflammation induced by cigarette smoke extracts in a human monocyte/macrophage cell line

QIN Yanqin, CHEN Yulong, ZHAO Peng, FENG Suxiang, WU Yaosong, LIU Xuefang, DONG Haoran, ZHENG Wanchun, MAO Xiaoning, LI Jiansheng   

  1. Henan Key Laboratory of Chinese Medicine for Respiratory Disease, Henan University of Chinese Medicine;Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of China, Henan University of Chinese Medicine;
  • Online:2021-06-03 Published:2021-06-03
  • Supported by:
    Supported by National Natural Science Fund of China (No.81130062, 81403367), the National Key Technology R&D Program during the 12th Five-Year Plan Period(2014BAI10B06)

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Abstract: OBJECTIVE: To study the mechanistic effects of Tiaobu Feishen therapy(TBFS) on inflammation induced by cigarette smoke extract(CSE) in a human monocyte/macrophage cell line.METHODS: The human monocyte/macrophage cell line THP-1 was stimulated with 10% CSE in the presence or absence of Bufei Yishen formula(BYF),Bufei Jianpi formula(BJF) and Yiqi Zishen formula(YZF). All formulations contained serum. Pro-inflammatory cytokines were measured in the supernatants using enzyme-linked immunosorbent assay.The activity of STAT3 DNA binding was detected using electrophoretic mobility shift assay and janus kinase/signal transducer and activator of transcription(JAK/STAT) pathway activation was assessed using Western blotting.RESULTS: The results showed that BYF, BJF and YZF treatment strongly decreased the CSE-induced secretion of interleukin(IL)-6, IL-8, tumor necrosis factor-α and matrix metalloproteinase-9 by THP-1 cells. Furthermore, BYF, BJF and YZF treatment attenuated STAT3 DNA binding capacity and JAK2 and STAT3 were shown to be phosphorylated.CONCLUSION: The data revealed that BYF, BJF and YZF effectively inhibited a CSE-induced inflammatory response in THP-1 cells by limiting activation of the JAK2/STAT3 pathway.

Key words: cigarette smoking, janus kinase 2, STAT3 transcription factor, THP-1 cells, matrix metalloproteinases, Tiaobu Feishen therapy

Cite this article

QIN Yanqin, CHEN Yulong, ZHAO Peng, FENG Suxiang, WU Yaosong, LIU Xuefang, DONG Haoran, ZHENG Wanchun, MAO Xiaoning, LI Jiansheng. Tiaobu Feishen therapy inhibits inflammation induced by cigarette smoke extracts in a human monocyte/macrophage cell line[J]. Journal of Traditional Chinese Medicine, 2021, 41(3): 360-366.