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Journal of Traditional Chinese Medicine ›› 2021, Vol. 41 ›› Issue (1): 51-58.DOI: 10.19852/j.cnki.jtcm.2021.01.007

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Efficacy of water fraction from Dioscorea cirrhosa on oxidative stress and apoptosis in H9c2 cardiomyocytes induced by H_2O_2

LIU Chunhua, LU Dingyan, YOU Jingrui, LU Yuan, SUN Jia, PAN Jie, WANG Yonglin, WANG Aimin, LAN Yanyu, LI Yongjun, LIU Ting   

  1. State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine (Ministry of Education), Guizhou Medical University;School of Pharmacy, Guizhou Medical University;Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University;
  • Online:2021-02-03 Published:2021-02-03
  • Supported by:
    Supported by National Natural Science Foundation of China Basic Research on Ethnopharmacology of Guizhou Karst Area (No. U1812403), Study on the Active Components of Anti-oxidative Stress Activities of Shengxiong Glucose Injection Based on Pi3k/akt Pathway (No. 81760699), Investigation of The Active Components and Mechanism of Polygonum Orientale Ameliorating Myocardial Ischemic Injury Based on"Components-Network Targets-Effects"(No.81803700), Central Guided Local Science and Technology Special Project Capability Enhancement of Guizhou Provincial Key Laboratory of Pharmaceutics (No. 20184006), Guizhou Provincial Natural Science Foundation Effects of Gerbera Piloselloides on Airway Inflammation in Bronchial Asthma and Its Medicinal Substance Basis (20181128), Study on the Molecular Mechanism and Active Ingredients of Polygonum Orientale in Treating Myocardial Ischemic Disease Based on Systemic Pharmacology Strategy (No. 20201z068), Guizhou Provincial People's Pharmacy Effective Material Basic Research Science and Technology Innovation Talent Team (No. 20165613), Cultivation of High-level Innovative Talents in Guizhou Province (Hundred Levels)(No. 20165677), Study on the Effective Component Group and Its Mechanism of Anti-myocardial Ischemia Injury of Polygonum Orientale Based on"Component-network Target"(No.20175718), Study on the Effective Material Basis of Shengxiong Glucose Injection for Its Anti-oxidative Stress Activities Mediated by Pi3k/akt (No. 20191280), Doctoral Start Fund of Guizhou Medical University Study on the Effective Material Basis of Gerbera Piloselloides Against Bronchial Asthma Based on In Vivo Processes (No. YJ2017-12)

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Abstract: OBJECTIVE: To investigate the efficacy of water fraction from Dioscorea cirrhosa(WF) on oxidative damage and apoptosis of cardiomyocytes induced by H_2O_2, and to study its mechanism.METHODS: Cell viability was measured by the MST assay kit. The content of malondialdehyde(MDA),release of lactate dehydrogenase(LDH) and activity of catalase(CAT) and superoxide dismutase(SOD)were detected by biochemical kit. The content of reactive oxygen species(ROS) was assessed by nonfluorescent probe 2′,7′-dichlorofluorescin diacetate(DCFH-DA). JC-1 was used to analyze the mitochondrial membrane potential(mtΔΨ) and Annexin-V-FITC/PI staining was applied to assess apoptosis of H9c2 by flow cytometry. Moreover, the expression of B-cell lymphoma-2(Bcl-2), Bcl-2-associated X(Bax), caspase-3, caspase-9, cleaved-caspase-3 and cleaved-caspase-9 proteins was determined by western blot analysis.RESULTS: WF increased cell viability and decreased LDH leakage in H9c2 cells exposed to H_2O_2. WF treatment decreased ROS and MDA level, enhanced SOD and CAT activities, improved mtΔΨand inhibited apoptosis. Western blot analysis demonstrated that the ratio of Bcl-2/Bax was increased and the expression cleaved-caspase-3, caspase-3,cleaved-caspase-9 and caspase-9 were decreased in group treated with WF.CONCLUSION: WF protects H9c2 myocardial cells on H_2O_2-induced oxidative stress and apoptosis by scavenging ROS, improving antioxidant capacity,protecting mitochondrial and regulating the proteins expression related to apoptosis.

Key words: Dioscorea, water fraction, hydrogen peroxide, oxidative stress, apoptosis, myocytes,cardiac

Cite this article

LIU Chunhua, LU Dingyan, YOU Jingrui, LU Yuan, SUN Jia, PAN Jie, WANG Yonglin, WANG Aimin, LAN Yanyu, LI Yongjun, LIU Ting. Efficacy of water fraction from Dioscorea cirrhosa on oxidative stress and apoptosis in H9c2 cardiomyocytes induced by H_2O_2[J]. Journal of Traditional Chinese Medicine, 2021, 41(1): 51-58.