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Journal of Traditional Chinese Medicine ›› 2021, Vol. 41 ›› Issue (1): 36-43.DOI: 10.19852/j.cnki.jtcm.2021.01.005

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Effect of extract from Yiyuan Yiliu Tang(益元抑瘤汤) on human lung adenocarcinoma and human hepatoma cell lines

YI Qingqing, CAI Weiwei, CHANG Qing, LIANG Dongyu, YANG Rong, JIANG Guowei, QIU Liying   

  1. Clinical Research Center, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences;Department of Basic Medicine,Wuxi Medical School, Jiangnan University;Pathology department, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences;Departments of Pharmacy, Jiading District Central Hospital Affiliated Shanghai University of Medicine& Health Sciences;
  • Online:2021-02-03 Published:2021-02-03
  • Supported by:
    Supported by Institutional Funding (No. JDKW-2020-0013,the Nature Science Foundation of Jiading District, Shanghai, No. 2020-ZD-03, the Key Projects of Jiading District Health Commission, Shanghai, No. JDKW-2018-W21, the Agricultural and Social Research Projects of Jiading, Shanghai, and No.2020-KY-14, the General project of Jiading District Health Commission, Shanghai)

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Abstract: OBJECTIVE: To investigate the efficacy of the extract from Yiyuan Yiliu Tang(益元抑瘤汤, YYYLT) on human lung adenocarcinoma cells A549 and human hepatoma cells Bel7402.METHODS: The cancer cell lines were treated with various concentrations(0, 100, 200, 300, and400 μg/m L) of the crude water extract of YYYLT and then cell viability, toxicity, cytokine secretion,and cell cycle/apoptosis were determined by MTT assay, LDH assay, and flow cytometry, respectively.RESULTS: The extract from YYYLT significantly suppressed the proliferation of the cancer cell lines and the release of interleukin-2 and tumor necrosis factor-α in a dose-dependent manner. The extract also promoted apoptosis, caused cell cycle arrest at G0/G1 phase, and increased the expression of caspase-3, B-cell lymphoma-2(Bcl-2), and Bcl-2-associated X proteins.CONCLUSION: The extract from YYYLT might be a potential treatment for human lung and liver cancers.

Key words: Antineoplastic agents, Cell cycle, Apoptosis, Nodal protein, Yiyuan Yiliu Tang

Cite this article

YI Qingqing, CAI Weiwei, CHANG Qing, LIANG Dongyu, YANG Rong, JIANG Guowei, QIU Liying. Effect of extract from Yiyuan Yiliu Tang(益元抑瘤汤) on human lung adenocarcinoma and human hepatoma cell lines[J]. Journal of Traditional Chinese Medicine, 2021, 41(1): 36-43.