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Journal of Traditional Chinese Medicine ›› 2020, Vol. 40 ›› Issue (3): 422-431.DOI: 10.19852/j.cnki.jtcm.2020.03.010

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Osteoking downregulates Mgp in an osteoporotic fracture rat model

Sun Yan, Wang Xiaoqi, Chen Ran, Zhu Di, Shen Zhiqiang, Zhao Hongbin, Lee Wenhui   

  1. Pharmaceutical College & Key Laboratory of Pharmacology for Natural Products of Yunnan Province, Kunming Medical University;Key Laboratory of Bio-active Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, Kunming Institute of Zoology;the Traumatology of Emergency Department, the First People's Hospital of Yunnan Province/the Affiliated Hospital of Kunming University of Science and Technology;the Laboratory Department, The Second Affiliated Hospital of Kunming Medical University;
  • Online:2020-06-15 Published:2020-06-15
  • Supported by:
    Supported by the National Science Foundation of China-Yunnan Province Joint Fund (The mechanisms of the Osteoking for promoting bone formation in OP and OPF,No.U1502227)

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Abstract: OBJECTIVE: To investigate the effectiveness of osteoking, a Traditional Chinese Medicine originating from Yi nationality, against osteoporosis(OP) and osteoporotic fracture(OPF), and to elucidate its mechanism of action.METHODS: An osteoporotic fracture rat model was established; animals were divided into three treatment groups: parathyroid hormone, osteoking and0.9%NaCl. After 4, 8 and 12 weeks of treatment, serum and bone tissues were collected. Enzyme-linked immuno sorbent assay, x-ray, histopathological evaluation and proteomics were used.Proteomics and GO annotation were performed based on identified peptides. The relative network was obtained from the STRING database and verified by polymerase chain reaction and Western blotting.RESULTS: After osteoking treatment, the bone mineral density(BMD) increased with time in the osteoking group. At week 12, the BMD and bone mineral salt content of the osteoking group were 4.5%and 20.6% higher than those of the negative control group, respectively. Furthermore, the body weight followed the order of positive control group > osteoking group > negative control group,with significant differences among the groups(P

Key words: Osteoporosis, Osteoporotic fractures, Matrix Gla protein, Osteoking

Cite this article

Sun Yan, Wang Xiaoqi, Chen Ran, Zhu Di, Shen Zhiqiang, Zhao Hongbin, Lee Wenhui. Osteoking downregulates Mgp in an osteoporotic fracture rat model[J]. Journal of Traditional Chinese Medicine, 2020, 40(3): 422-431.