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Journal of Traditional Chinese Medicine ›› 2020, Vol. 40 ›› Issue (2): 204-211.DOI: 10.19852/j.cnki.jtcm.2020.02.004

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Notch signaling pathway mediates the immunomodulatory mechanism of Yangfei Huoxue decoction alleviating bleomycin-induced pulmonary fibrosis in rats

Chen Hui, Liu Xiao, Su Yinxu, Chang Ke, Wu Haotian, Su Guangbao, Gong Jiening   

  1. Basic Medical College, Nanjing University of Chinese Medicine;Pediatrics, Chengdu University of Traditional Chinese Medicine;Traditional Chinese Medicine Department, Baoji People's Hospital;
  • Online:2020-04-15 Published:2020-04-15
  • Supported by:
    Supported by the National Natural Science Foundation of China (Based on Notch Signaling Pathway and Its Epigenetic Changes to Discuss the Mechanism of Nourishing Lung and Promoting Blood Circulation Formula to Prevent and Treat Pulmonary Fibrosis,No. 81373603)

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Abstract: OBJECTIVE: To investigate the immunomodulatory mechanism by which Yangfei Huoxue decoction(YHD) alleviates bleomycin(BLM)-induced pulmonary fibrosis(PF) in rats.METHODS: Rats were randomly divided into two time-point groups(day 14 and 28), and each timepoint group comprised the following six subgroups: control, BLM, dexamethasone(DXM), YHD high dose(YHD-H), YHD middle dose(YHD-M), and YHD low dose(YHD-L). Haematoxylin and eosin and Masson staining, flow cytometry, enzymelinked immunosorbent assay, Western blotting and UPLC-QT of analyses were examined.RESULTS: The results showed that YHD reduced the degree of alveolar inflammation and fibrosis;downregulated the expression of CD28, CD80,CD86, Delta-like 1, Notch2, and Notch3; and upregulated the proportions of Th1/Th2 and Tc1/Tc2. The seven components of YHD were detected.CONCLUSION: The current study indicates that YHD mainly functions by regulating the immune system and that the molecular mechanism may be related to the regulation of the Notch signaling pathway.

Key words: Pulmonary fibrosis, Immunity, Receptors,Notch, Signal transduction, Th1-Th2 balance, Yangfei Huoxue decoction

Cite this article

Chen Hui, Liu Xiao, Su Yinxu, Chang Ke, Wu Haotian, Su Guangbao, Gong Jiening. Notch signaling pathway mediates the immunomodulatory mechanism of Yangfei Huoxue decoction alleviating bleomycin-induced pulmonary fibrosis in rats[J]. Journal of Traditional Chinese Medicine, 2020, 40(2): 204-211.