Advanced Search

Journal of Traditional Chinese Medicine ›› 2019, Vol. 39 ›› Issue (06): 875-884.DOI: 10.19852/j.cnki.jtcm.2019.06.015

Previous Articles     Next Articles

Rongchang and Xifeng capsules suppress pentylenetetrazole-induced seizures and change rest/wake behavior in zebrafish larvae

Zhang Shuhui, Xu Jia, Wang Chao, Feng Daofu, Dai Liangti, Kuang Xiangyu, Zhu Shuyi, Fang Yongchun, Chen Dongyan, He Xianghui, Feng Xizeng   

  1. State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education;College of Life Science, Nankai University;Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Department of Histology and Embryology, School of Medicine,Nankai University;The Institute of Robotics and Automatic Information Systems, Tianjin Key Laboratory of Intelligent Robotics, Nankai University;Department of General Surgery,Tianjin Medical University General Hospital;
  • Online:2019-12-15 Published:2019-12-15
  • Supported by:
    Supported by the Chinese National Natural Science Foundation of China:In situ microscopic Analysis and manipulator for Life Science(No.61327802); the Chinese National Natural Science Foundation of China:Functional Nanoparticle Drug Vector for Zebrafish Development and Biocompatible Assessmen(No.81501589);the Chinese National Natural Science Foundation of China:Development of a cross-scale fast AFM system for life science(No.61127006);the Chinese National Natural Science Foundation of China:Study on the Method of Automatic Nano-manipulation for Cell-Oriented Accurate Displacement(No.61633012), the Tianjin Science Technology Research Funds of China:the function of Rab23 gene in zebrafish development(No.14JCQNJC09600), the National Basic Research Program of China:The Basic and Frontier of the New Topological Structure of the Molecular-based Functional Carbon Materials(No.2015CB856500)

PDF

296

Abstract: OBJECTIVE: To evaluate the effects of Rongchang capsule and Xifeng capsule on pentylenetetrazoleinduced epilepsy in zebrafish larvae and to explore the possible mechanisms behind their actions.METHODS: We utilized a trajectory tracking system to monitor seizures in zebrafish larva to confirm that certain concentrations of Rongchang capsule and Xifeng capsule produce antiepileptic effects.c-fos expression was assessed by quantitative reverse transcription-polymerase chain reaction to validate the efficacy of the capsules. Rest/wake behavior and correlation analysis predicted the targets of Rongchang capsule and Xifeng capsule.RESULTS: Larval movement times and total distances traveled by zebrafish larvae experiencing pentylenetetrazole(PTZ)-induced seizures were decreasedbyvalproatetreatment.Rongchang(500 μg/m L) and Xifeng(200 μg/m L) rescued the epileptic behaviors and down-regulated c-fos expression in the brains of larvae, which indicated antiepileptic effects. The rest/wake behavioral profiles showed that Rongchang and Xifeng differentially decreased rest time at night and increased larval locomotor activities during the day. Based on correlation between the actions of the two capsules and known compounds, we predicted that they might change rest/wake behaviors by affecting serotonin, GABAergic and histamine signaling pathways.CONCLUSION: The efficacy of Rongchang capsule and Xifeng capsule in alleviating epilepsy-like behaviors and molecular responses was confirmed.Our study provides insight into the capsules' effect on epilepsy.

Key words: Zebrafish, Larva, Anticonvulsants, Rongchang capsule, Xifeng capsule, Rest/wake behavior

Cite this article

Zhang Shuhui, Xu Jia, Wang Chao, Feng Daofu, Dai Liangti, Kuang Xiangyu, Zhu Shuyi, Fang Yongchun, Chen Dongyan, He Xianghui, Feng Xizeng. Rongchang and Xifeng capsules suppress pentylenetetrazole-induced seizures and change rest/wake behavior in zebrafish larvae[J]. Journal of Traditional Chinese Medicine, 2019, 39(06): 875-884.