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Journal of Traditional Chinese Medicine ›› 2019, Vol. 39 ›› Issue (05): 609-623.DOI: 10.19852/j.cnki.jtcm.2019.05.002

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In vitro study of the nephrotoxicity of total Dahuang(Radix Et Rhizoma Rhei Palmati) anthraquinones and emodin in monolayer human proximal tubular epithelial cells cultured in a transwell chamber

Cao Chunyu, Hui Lianqiang, Li Chun, Yang Yifei, Zhang Jiyuan, Liu Ting, Hao Ran, Zhang Yi   

  1. Safety Evaluation Center for Chinese Materia Medica, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;Quality Standard Center for Chinese Materia Medica, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;Quality Standard Center for Chinese Materia Medica, Capital Medical University;Department of Sciences and Education, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;Pharmacology Research Center for Chinese Materia Medica, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;
  • Online:2019-10-15 Published:2019-10-15
  • Supported by:
    Supported by the Central Public Welfare Research Institutes Foundation-funded Project:A new research of renal toxicity early detection of Chinese medicine(ZZ0908035), Establishment of HK-2 cell monolayer model of human proximal renal tubular epithelial cells(ZXKT15022), Bioactivity based quality control for Chinese herbal medicine using Rhubarb as model system(GH2017-01-02), Natural Science Foundation-funded Project:Study on the bacterial endotoxin detection method of traditional Chinese medicine injections(90709043)

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Abstract: OBJECTIVE: To examine changes in the morphology and physiological functions of human proximal tubular epithelial cells(HK-2 cells) caused by total Dahuang(Radix Et Rhizoma Rhei Palmati) anthraquinones(TDA) and emodin.METHODS: HK-2 cells were cultured on polycarbonate(PCF) membranes to form a complete monolayer of cells.A fluorescein isothiocyanatedextran(FITC) permeability assay was conducted and secretion of γ-glutamyltranspeptidase(GGT),lactate dehydrogenase(LDH), N-acetyl-β-D-glucosaminidase(NAG) and kidney injury molecule 1(KIM-1) was examined.The reabsorption of glucose and the excretion of para-aminohippuric acid(PAH)by HK-2 cells were also examined.The morphology of HK-2 cells was observed using optical microscopy and scanning electron microscopy.The cytoskeleton of HK-2 cells was observed under a fluorescence microscope.RESULTS: Compared with the results for the dimethyl sulfoxide group, treatment of cells with TDA and emodin showed statistically significant differences in the FITC leakage rate, the apical/basolateral ratio of LDH and GGT, and the secretion of GGT,LDH, NAG and KIM-1.At 64 μg/mL, TDA markedly inhibited blood glucose reabsorption and remarkably suppressed PAH excretion by HK-2 cells.Both TDA and emodin caused various degrees of damage to the morphology and cytoskeleton of HK-2 cells with the degree of damage correlating positively with the dosage of the tested substances.CONCLUSION: Both TDA and emodin caused damage to human renal proximal tubular epithelial cells at certain dosages.At the same dosage, TDA caused more severe damage than emodin to the HK-2 cells.

Key words: Anthraquinones, Rheum, Emodin, Nephrotoxicity, Human proximal tubular epithelial cell, Transwell chamber

Cite this article

Cao Chunyu, Hui Lianqiang, Li Chun, Yang Yifei, Zhang Jiyuan, Liu Ting, Hao Ran, Zhang Yi. In vitro study of the nephrotoxicity of total Dahuang(Radix Et Rhizoma Rhei Palmati) anthraquinones and emodin in monolayer human proximal tubular epithelial cells cultured in a transwell chamber[J]. Journal of Traditional Chinese Medicine, 2019, 39(05): 609-623.