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Journal of Traditional Chinese Medicine ›› 2019, Vol. 39 ›› Issue (01): 45-49.DOI: 10.19852/j.cnki.jtcm.2019.01.005

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Three Tiaobu Feishen therapies protect human alveolar epithelial cells against cigarette smoking and tumor necrosis factor-mlear factor-kappa B pathwayα-induced inflamation by nuc

Chen Yulong, Wu Yaosong, Li Jiansheng, Feng Suxiang, Hao Lili, Liu Xuefang, Zheng Wanchun, Dong Haoran, Qin Yanqin, Yin Sugai, Zhao Peng   

  1. Henan Key Laboratory of Chinese Medicine for Respiratory Disease, Henan University of Chinese Medicine;Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Chinese Medicine;
  • Online:2019-02-15 Published:2019-02-15
  • Supported by:
    National Natural Science Fund of China(No.81130062,No.81403367), Outstanding Traditional Chinese Medicine Academic Leader Program of Henan Province(No.HNZYLJ201301001), the National Key Technology R&D Program during the 12th Five-Year Plan Period(No.2014BAI10B06)

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Abstract: OBJECTIVE: To investigate the efficacy of Tiaobu Feishen formulae(TBFS), including Bufei Jianpi formula(BJF), Bufei Yishen formula(BYF), and Yiqi Zishen formula(YZF), on inflammatory response, protease-anti-protease imbalance and collagen deposition in rats.METHODS: In present work, we used an in vitro model of cigarette smoking extract(CSE)-and tumor necrosis factor-α(TNF-α)-induced A549 cellsto examine the efficacy of BJF, BYF and YZF on the production of inflammatory cytokines, including TNF-α and interleukin(IL)-8, IL-6, matrix metalloproteinases(MMP)-9, and IL-10 in CSE or TNF-ls. And their related transcripα-induced A549 celtion factors and signaling pathway were also analyzed.RESULTS: The results showed that BJF, BYF and YZF could significantly decrease the expression levels of the pro-inflammatory cytokines induced by CSE or TNF-α. Furthermore, BJF, BYF and YZF could suppress CSE-or TNF-α-induced activation of nuclear factor-kappa B(NF-κB) transcription factors and its corresponding pathways. Taken together, these data implied that BJF, BYF and YZF effectively inhibited CSE-or TNF-α-induced inflammatory response in alveolar epithelial cell, which was due to their inhibition effect on NF-κB pathways.CONCLUSION: Our findings suggest that the Tiaobu Feishen therapies may protect human alveolar epithelial cells against cigarette smoking and TNF-α-induced inflammation. NF-κB pathway may involve in the actions.

Key words: Chinese medical formula, Cigarette smoking, Tumor necrosis factor-alpha, A549 cells

Cite this article

Chen Yulong, Wu Yaosong, Li Jiansheng, Feng Suxiang, Hao Lili, Liu Xuefang, Zheng Wanchun, Dong Haoran, Qin Yanqin, Yin Sugai, Zhao Peng. Three Tiaobu Feishen therapies protect human alveolar epithelial cells against cigarette smoking and tumor necrosis factor-mlear factor-kappa B pathwayα-induced inflamation by nuc[J]. Journal of Traditional Chinese Medicine, 2019, 39(01): 45-49.