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Journal of Traditional Chinese Medicine ›› 2013, Vol. 33 ›› Issue (04): 486-491.DOI: 10.1016/s0254-6272(13)60153-3

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Relationship between iNOS expression and apoptosis in cerebral tissue, and the effect of Sini injection in endotoxin shock rats

Hui Pei, Yuzhong Zhang, Haiyan Wu, Liwei Ren, Xu Jia, Yinzhu Zhang, Ming Chen   

  1. Xiyuan Hospital of China Academy of Chinese Medical Sciences;College of Preclinical Medicine, Beijing University of Chinese Medicine;
  • Accepted:2013-08-15 Online:2013-08-15 Published:2013-08-15

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Abstract: OBJECTIVE: To investigate the dynamic changes and relationship of inducible nitric oxide synthase (iNOS) and apoptosis in endotoxin shock rats, as well as the effects of Sini injection. METHODS: In total, 102 Sprague-Dawley (SD) rats were randomly divided into a normal group (n=6, NG), sham operation group (n=24, OG), model group (n=24, MG), dexamethasone group (n=24, DG), and Sini group (n=24, SG). The endotoxin shock model was induced by an intravenous injection of lipopolysaccharide (LPS) (8 mg/kg). Rats in the OG, MG, DG, and SG groups were further divided into 4 groups: 1, 2, 3 and 6 h after shock groups (n=6 per group). iNOS expression was detected by immunohistochemistry. Terminal Deoxynucleotidyl Transferase Mediated Deoxyuridine Triphosphate-biotin Nick End Labeling was employed to measure apoptosis. RESULTS: No iNOS expression was found in the OG group. Compared with the OG group, iNOS expres-sion in the MG group was markedly elevated, reached a peak at 1 h (P

Key words: Septic shock, Nitric oxide synthase type II, Apoptosis, Sini injection

Cite this article

Hui Pei, Yuzhong Zhang, Haiyan Wu, Liwei Ren, Xu Jia, Yinzhu Zhang, Ming Chen. Relationship between iNOS expression and apoptosis in cerebral tissue, and the effect of Sini injection in endotoxin shock rats[J]. Journal of Traditional Chinese Medicine, 2013, 33(04): 486-491.