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Journal of Traditional Chinese Medicine ›› 2012, Vol. 32 ›› Issue (03): 404-410.DOI: 10.1016/s0254-6272(13)60046-1

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In vitro inhibitory and pro-apoptotic effect of Stellera Chamaejasme LExtract on human lung cancer cell line NCI-H157

Liu, Xiaoni; Li, Yujie; Yang, Qing; Chen, Ying; Weng, Xiaogang; Wang, Yiwei; Li, Ning; Zhu, Xiaoxin   

  1. China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China; Capital Med Univ, Beijing YouAn Hosp, Beijing 100069, Peoples R China; Inst Liver Dis, Beijing 100069, Peoples R China
  • Accepted:2012-09-15 Online:2012-09-15 Published:2012-09-15

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Abstract: OBJECTIVE:To investigate the inhibitory and pro-apoptotic effect of Stellera Chamaejasme L extract(ESC) in vitro.METHODS:ESC was first extracted with ethanol,and then washed using a polyamide column with 60% ethanol.ESC was then decompressively recycled and vacuum dried at room temperature to obtain active fractions.Subsequently,the cytotoxic and apoptotic effects of ESC on NCI-H157 human lung cancer cells were determined.RESULTS:The results showed that ESC was rich in isomers of Chamaejasminor,neochamaejasmine and Sikokianin.ESC had significant cytotoxicity against NCI-H157 cells,with an IC 50 of approximately 18.50 μg.mL-1.ESC caused significant increase in total apoptotic rate,the activity of caspase 3 and 8,and Fas protein expression(P

Key words: Stellera chamaejasme L, Lung neoplasms, Apoptosis, Antigens, CD95, Lissamine rhodamine B

Cite this article

Liu, Xiaoni; Li, Yujie; Yang, Qing; Chen, Ying; Weng, Xiaogang; Wang, Yiwei; Li, Ning; Zhu, Xiaoxin. In vitro inhibitory and pro-apoptotic effect of Stellera Chamaejasme LExtract on human lung cancer cell line NCI-H157[J]. Journal of Traditional Chinese Medicine, 2012, 32(03): 404-410.