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Journal of Traditional Chinese Medicine ›› 2014, Vol. 34 ›› Issue (03): 362-368.DOI: 10.1016/S0254-6272(14)60103-5

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Effect of Honghua (Flos Carthami) on nitric oxide production in RAW 264.7 cells and α-glucosidase activity

Hui Liao, Linda Banbury, Hongping Liang, Xiaomin Wang, Xiaokai Lü, Ling Hu, Jin Wu   

  1. Department of Pharmacy, Shanxi Provincial People's Hospital;Southern Cross Plant Science, Southern Cross University;Department of Clinical Laboratory, Shanxi Provincial People's Hospital;Department of Pharmacy,Shanxi DaYi Hospital;Department of Endocrinology, Shanxi Provincial People's Hospital;Management of Hospital, Shanxi Medical University Second Hospital;
  • Accepted:2014-06-15 Online:2014-06-15 Published:2022-04-05

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Abstract: OBJECTIVE: To study the effects of extracts from Honghua(Flos Carthami) on lipopolysaccharide induced nitric oxide(NO) production in RAW 264.7cells and the influence of the extracts on yeastα-glucosidase activity. The total flavonoid content of the extracts was also determined.METHODS: Cytotoxicity of the extracts to RAW264.7 cells was evaluated by the ATPlite? method.Inhibitory effects of the extracts on NO production were evaluated by Griess assay. Curcumin was used as a positive control. Screening of extracts for potential α-glucosidase inhibitors was done by a fluorometric assay.The assay was based on the hydrolysis of 4-methylumbelliferyl-a-D-glucopyranoside to form the fluorescent product, 4-methylumbelliferone. Acarbose was used as a positive control. The total flavonoid content was tested using kaempferol as the standard.RESULTS: There were significant inhibitory effects on NO production when the extracts were 25-100μg/mL(P

Key words: Carthamus tinctorius, Nitric oxide, Alpha-glucosidases, Flavonoids, RAW264.7cells

Cite this article

Hui Liao, Linda Banbury, Hongping Liang, Xiaomin Wang, Xiaokai Lü, Ling Hu, Jin Wu. Effect of Honghua (Flos Carthami) on nitric oxide production in RAW 264.7 cells and α-glucosidase activity[J]. Journal of Traditional Chinese Medicine, 2014, 34(03): 362-368.