Advanced Search

Journal of Traditional Chinese Medicine ›› 2014, Vol. 34 ›› Issue (03): 302-308.DOI: 10.1016/S0254-6272(14)60094-7

Previous Articles     Next Articles

Effect of berberine on activity and mRNA expression of N-acetyl transferase in human lung cancer cell line A549

Shengyan Xi, Kunhan Chuang, Kwangming Fang, Yimin Lee, Jinggung Chung, Yaochen Chuang   

  1. Department of Traditional Chinese Medicine,Medical College of Xiamen University;Cancer Research Center of Xiamen University;Department of Applied Cosmetology,Hwa Hsia Institute of Technology;Department of Dental Technology and Materials Science, Central Taiwan University of Science and Technology;Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology;Graduate Institute of Biological Science and Technology, China Medical University;Center of General Education, Central Taiwan University of Science and Technology;Chinaand Kiang Wu Nursing College of Macau;
  • Accepted:2014-06-15 Online:2014-06-15 Published:2022-04-05

PDF

127

Abstract: OBJECTIVE:Tostudy the effects of berberine on activity and mRNA expression of N-acetyltransferase in human lung cancer cell lineA549.METHODS: N-acetyltransferase antibodies were prepared. The human lung cancer A549 cells were cultivated randomly in the wells of culture plate,and divided into the control group, and berberine0.0008, 0.008, 0.08, 0.8 and 1.6 mM treatment groups,with 3 wells for each group.24h later,A549 cells in each group were collected respectively, the content of N-acetyltransferase was detected by Flow cytometry, and the mRNA expression of N-acetyltransferase was observed by reverse transcription polymerase chain reaction.RESULTS: The N-acetyltransferase content in human lung cancer A549 cells decreased with the increasing of berberine concentration, significantly lower than that in the control group(P

Key words: Berberine, Acetyltransferases, Cell line, tumor, Neoplasms

Cite this article

Shengyan Xi, Kunhan Chuang, Kwangming Fang, Yimin Lee, Jinggung Chung, Yaochen Chuang. Effect of berberine on activity and mRNA expression of N-acetyl transferase in human lung cancer cell line A549[J]. Journal of Traditional Chinese Medicine, 2014, 34(03): 302-308.